Determination of haplotypes at structurally complex regions using emulsion haplotype fusion PCR
نویسندگان
چکیده
منابع مشابه
Long-range, high-throughput haplotype determination via haplotype-fusion PCR and ligation haplotyping
Ligation Haplotyping is a robust, novel method for experimental determination of haplotypes over long distances, which can be applied to assaying both sequence and structural variation. The simplicity and efficacy of the method for genotyping large chromosomal rearrangements and haplotyping SNPs over long distances make it a valuable and powerful addition to the methodological repertoire, which...
متن کاملMolecular haplotyping by linking emulsion PCR: analysis of paraoxonase 1 haplotypes and phenotypes
Linking emulsion PCR (LE-PCR) enables formation of minichromosomes preserving phase information of two polymorphic loci, hence the haplotype. Emulsion PCR confines two amplicons of two linked polymorphic sites on a single template molecule to one aqueous-phase droplet. Linking PCR uses biotinylated, overlapping linking primers to connect these amplicons in the droplet. After LE-PCR, unlinked am...
متن کاملHaplotype inference for present-absent genotype data using previously identified haplotypes and haplotype patterns
MOTIVATION Killer immunoglobulin-like receptor (KIR) genes vary considerably in their presence or absence on a specific regional haplotype. Because presence or absence of these genes is largely detected using locus-specific genotyping technology, the distinction between homozygosity and hemizygosity is often ambiguous. The performance of methods for haplotype inference (e.g. PL-EM, PHASE) for K...
متن کاملLaboratory Scale Determination of Optimum Flotation Conditions of Chalcocite at the Sarcheshmeh Copper Complex Using the Taguchi Method
Process optimization is one of the most important activities in today's competitive industries. the rather high cost of research and development has necessitated the development of experimental methods by which the factors affecting processes
could be determined with minimum number of experiments. Over the last two decades, various types of experimental designs have been used. Among the diff...
متن کاملLaboratory Scale Determination of Optimum Flotation Conditions of Chalcocite at the Sarcheshmeh Copper Complex Using the Taguchi Method
Process optimization is one of the most important activities in todays competitive industries. the rather high cost of research and development has necessitated the development of experimental methods by which the factors affecting processes could be determined with minimum number of experiments. Over the last two decades, various types of experimental designs have been used. Among the differe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Genomics
سال: 2012
ISSN: 1471-2164
DOI: 10.1186/1471-2164-13-693